锘海LS18系列平鋪光片顯微鏡   

? 組織更大? 看得更清 ? 成像更快 ? 活體可用 ?3D成像

锘海LS18平鋪光片顯微鏡采用平鋪虛擬掃描光片技術(shù)，克服了傳統(tǒng)光片顯微鏡中三維空間分辨率、信噪比和成像視野間的矛盾，可進(jìn)行更高分辨率的三維成像。

通過平鋪技術(shù)可對光片狀態(tài)進(jìn)行實(shí)時調(diào)節(jié)和自動校準(zhǔn)，保證其在不同應(yīng)用中的易用性和穩(wěn)定性。此外，根據(jù)不同樣品的特性，LS18還分別提供了優(yōu)化配件以及流程方案，方便使用者調(diào)整成像分辨率、速度、放大倍數(shù)等參數(shù)以及樣本的處理方法，以獲得微米級、亞微米級甚至百納米級三維空間分辨率的多色組織結(jié)構(gòu)信息。配套的三維圖像分析軟件和常用數(shù)據(jù)分析方案讓使用者可更高效地對結(jié)果進(jìn)行分析和解讀，因此LS18被廣泛應(yīng)用于發(fā)育、腫瘤、神經(jīng)等基礎(chǔ)生物科研領(lǐng)域以及藥物開發(fā)、藥效檢測、臨床病理檢測等醫(yī)藥領(lǐng)域。

應(yīng)用范圍：

生命科學(xué)：

?神經(jīng)科學(xué)   ?發(fā)育生物學(xué)   ?植物學(xué)   ?海洋生物學(xué)   ?細(xì)胞生物學(xué)   ?微生物學(xué)

基礎(chǔ)醫(yī)學(xué)與臨床醫(yī)學(xué)：

?心腦血管研究    ?腫瘤與病理   ?消化系統(tǒng)疾病   ?泌尿與生殖   ?肥胖與健康 ?骨科   ?眼科學(xué)   ?耳科學(xué)   ?口腔科學(xué)   ?皮膚科學(xué)   ?免疫與感染   ?藥理與毒理

生物技術(shù)與轉(zhuǎn)化醫(yī)學(xué)：

?類器官研究   ?藥物篩選與評價   ?3D生物打印

2025（18篇）

Nature Communications

Migration of CD8+ TSCM cells into intestine via PPBP-CXC axis increases host stress susceptibility by inhibiting gut microbiome-derived homovanillic acid

Cell Metabolism

Galnt2 neurons in the ventromedialhypothalamus counterregulatehypoglycemia via a brain-liver neurocircuit

Signal Transduction and Targeted Therapy

Rapid amyloid-β clearance and cognitive recovery through multivalent modulation of blood–brain barrier transport

Advanced Science

Mesenchymal Stromal Cells Play an Analgesic Role Through a Npy2r Sensory Neuron-Mediated Lung-to-Brain Axis

Brain, Behavior, and Immunity

DCX+ cells cripple systemic spleen immunity and promote tumor progression

EMBO Molecular Medicine

Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

Science Translational Medicine

Targeted suppression of glioma by ultralow-dose x-ray–induced photodynamic therapy and gold-based nanoclusters in preclinical models

作者：Shi Li, Shileng Chen, Yimin Li, Wenjing Sun, Junjun Li, Huaping Deng, Haizhen Ding,Zongzhang Wang, Luchao Zhu, Jun Liu, Minjie Wang, Yushuo Feng, Yuanyuan Xie,Yiru Wang, Yaqing Liu, Wenle Li, Qin Lin, Xiaobing Jiang, Hongmin Chen

Scientific Reports

Application of HEMA-AAm copolymer to achieve faster optical tissue transparency for 2D/3D fluorescence imaging

作者：Ma, Ruixiu Qin, Qiufeng Yao, Yier Li, Xingshun Cong, Wenhui Wu, Qi Zhao, Hua Ye& Kefeng Wu

eLife

3D reconstruction of neuronal allometry and neuromuscular projections in asexual planarians using expansion tiling light sheet microscopy

作者：Jing Lu, Hao Xu, Dongyue Wang, Yanlu Chen, Takeshi Inoue,Liang Gao, Kai Lei

Cell Regeneration

Morphological segmentation with tiling light sheet microscopy to quantitatively analyze the three?dimensional structures of spinal motoneurons

作者：Huijie Hu , Dongyue Wang, Yanlu Chen and Liang Gao

Nature Biomedical Engineering

Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes

作者：Jianfeng Dai1, Weijun Wei , Chenxu Yan, Ding-Kun Ji, Caiqi Liu,Jialiang Huang, Chenyi Liang, Jianjun Liu, Zhiqian Guo&Wei-Hong Zhu

Nature Photonics

Curved light sheet microscopy for centimetre-scale cleared tissue imaging

作者：Lijuan Tang, Jiayu Wang , Jiayi Ding, Junyou Sun, Xing-jun Chen,Quqing Shen, Ruiheng Song, Peng Cao , Rong Gong, Fang Xu ,Woo-ping Ge , Wenzhi Sun , Hu Zhao & Jianglai Wu

Frontiers in Aging Neuroscience

Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson’s disease induced by 6-OHDA

作者：Mengqi Wang, Linglong Xiao, Yifeng Shi, Yaping Wu,Xinyuejia Huang, Yang Wu, Yangyang Xu, Lin Bai, Wei Pan,

Jie Zhang and Wei Wang

Cell Reports

Halting hepatocellular carcinoma: Identifying intercellular crosstalk in HBV-driven disease

作者：Lingyun Zhou, Chang-Hai Liu,Duoduo Lv, Binru Cai, Yiguo Hu,Manuel Romero-Go′ mez

Journal of Neuroimmunology

3D imaging and pathological analysis of microglia in LPS-treated mice with light-sheet fluorescence microscopy

作者：Renjie Liu, Jianbo Xiu

Nature Communications

Mitochondria transplantation transiently rescues cerebellar neurodegeneration improving mitochondrial function and reducing mitophagy in mice

作者：Shu-Jiao Li, Qian-Wen Zheng, Jie Zheng, Jin-Bao Zhang, Hui Liu,Jing-Jing Tie, Kun-Long Zhang, Fei-Fei Wu, Xiao-Dong Li, Shuai Zhang,Xin Sun, Yan-Ling Yang& Ya-YunWang

Nature Communications

Noradrenergic inputs from the locus coeruleus to anterior piriform cortex and theolfactory bulb modulate olfactory outputs

作者：Chi Geng, Ruochen Li, Shan Li,Penglai Liu, Yuxin Peng, Changyu Liu,Zhen Wang, Hongxing Zhang& Anan Li

Nature Communications

Noradrenergic inputs from the locus coeruleus to anterior piriform cortex and theolfactory bulb modulate olfactory outputs

2024（15篇）

Nature Communications

Charge-assisted stabilization of lipid nanoparticles enables inhaled mRNA delivery for mucosal vaccination

作者：Shuai Liu, Yixing Wen, Xinzhu Shan, Xinghuan Ma, Chen Yang, Xingdi Cheng, Yuanyuan Zhao, Jingjiao Li, Shiwei Mi, Haonan Huo,Wei Li, Ziqiong Jiang, Yijia Li, Jiaqi Lin, LeiMiao & Xueguang Lu

Signal Transduction and Targeted Therapy

EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammation

作者：Yingnan Bai'g, iming Chen’, Fanghao Guo?3, jinghong Zhang’, jinlin Hu", xuefei Tao", Qing Lu', Wenyi L?, Xueying Chen'Ting Gong', Nan Qiu', Yawei jin', Lifan Yang', Yu Lei, chengchao Ruan of, Qing jing*, john p Cooke , shijun wang 1og,Yunzeng Zou b.11@and Junbo Ge

Advanced Science

Whole-Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy

作者：Yezhou Wang, ZileJiang, Kai Zhang, Huimin Tang, Guimei Wang, Jinshan Gao,Guanghui He, Baoyue Liang, Li Li, Chaoyong Yang,* and Xianming Deng

Original Article

3D visualization of cellular and molecular distributions in human crystalline lenses at different age

作者：Wei Hu, Xiaoyu Tong, Lu Zhang, Jiajia Li, Kaijing Zhou, Wenhan Lu, Jiemei Shi, Wenjing Wang, Mengdi Zhang, Yicong Wang, Wendong Han, Liyipeng Chen, Jin Li, Yi Feng

鏈接：https://doi.0rg/10.62347/JAM06905

experimental eye research

A Novel Three-dimensional Method for Detailed Analysis of RGC Central Projections under Acute Ocular Hypertension

作者：Wenhan Lu, Yu Wang, Wei Hu , Xinyi Lin , Xiaoyu Tong , Yi Tian a,c , Yuning Chen , Yicong Wang , Yan Xiao  , Hongfang Yang , Yi Feng , Xinghuai Sun ,

advanced MATERIALS

Molecular Imaging of Ovarian Follicles and Tumors With Near-Infrared II Bioconjugates

作者：Yicong Wang, Wenhan Lu, Zi-Han Chen, Yan Xiao, Yu Wang, Wenhao Gao, Zhiming Wang, Ruihu Song, Zhao Fang, Wei Hu, Xiaoyu Tong, Kuinyu Lee, Zhenle Pei, Minzhen Xu, Fan Zhang, Hao Chen, Yi Feng

Neuron

Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson’s and Alzheimer’s disease co-pathology and behavior impairments

作者：Jie Xiang, Jingrong Tang, Fei Kang, Jiajun Ye, Yueying Cui, Zhentao Zhang, Jing Wang, Shengxi Wu, and Keqiang Ye

American Journal of Physiology-Endocrinology and Metabolism

Characterization of a novel adipose tissue located between abdominal lymph nodes and cervix/prostate in mice

作者：Lihao Hu, Gaochen Zhang, Xiaoyu Tong, Lulu Wang, Xiang Qiu, Hongbo Yang, Xinmei Liu, and Hefeng Huang

Research Square（預(yù)印本）

Astrocytic Bestrophin1 in the Anterior Cingulate Cortex Modulates the Formation and Persistence of Morphine Addiction Memory

作者：Linlin Sun, Zepeng Liu, Yifan Li, Xiaorui Lin, Yu Huo, Shengyang Guo, Xiang Fan, Xinzhi Ye, Yun Yao, Jiao Liu, Ning Wang, Sen Song, and Yijing Li

Science Advances

Netrin-1–engineered endothelial cell exosomes induce the formation of pre-regenerative niche to accelerate peripheral nerve repair

作者：Jinsheng Huang, Jiangnan Li , Senrui Li , Xiaoqi Yang, Nianci Huo, Qiang Chen, Wengang Wang, Ningning Yang , Yuanyi Wang  , and Nan Zhou

Nature Neuroscience

Somatosensory cortex and central amygdala regulate neuropathic pain-mediated peripheral immune response via vagal projections to the spleen

作者：Xia Zhu, Ji-Ye Huang, Wan-Ying Dong, Hao-Di Tang, Si Xu, Qielan Wu, Huimin Zhang, Ping-Kai Cheng, Yuxin Jin, Meng-Yu Zhu, Wan Zhao, Yu Mao, Haitao Wang, Yan Zhang, Hao Wang, Wenjuan Tao , Yanghua Tian , Li Bai & Zhi Zhang?

Science of The Total Environment

Kidney injury contributes to edema of zebrafish larvae caused by quantum dots

作者：Luanjin Wang , Cixin Liang , Naying Zheng , Chunyan Yang , Sen Yan , Xiang Wang , Zhenghong Zuo, Chengyong He

鏈接：https://doi.org/10.1016/j.scitotenv.2023.168420

nature communications

Alternations in inflammatory macrophageniche drive phenotypic and functionalplasticity of Kupffer cells

作者：Han-Ying Huang, Yan-Zhou Chen, Chuang Zhao, Xin-Nan Zheng, Kai Yu, Jia-Xing Yue, Huai-Qiang Ju, Yan-Xia Shi & Lin Tian

Journal of Ethnopharmacology

Investigating the pharmacological mechanism of Zhengyuan jiaonang for treating colorectal cancer via network pharmacology analysis and experimental verification

Nature Communications

Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells

2023（13篇）

Research

Characteristic Features of Deep Brain Lymphatic Vessels and Their Regulation by Chronic Stress

作者：Junzhuang Chang, Bingqing Guo, Yan Gao, Wei Li, Xiaoyu Tong, Yi Feng, and Nashat Abumaria

Nature Metabolism

Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice

作者：Junkun Jiang, Donglei Zhou, Anke Zhang, Wenjing Yu, Lei Du Huiwen Yuan, Chuan Zhang, Zelin Wang, Xuyang Jia Zhen-Ning Zhang & Bing Luant

Journal of Experimental & Clinical Cancer Research

Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinoma

作者：Lingyun Zhou, Linye He, Chang?Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin, Kunlin Xie, Javier Ampuero, Zhihui Li, Duoduo Lv, Miao Liu, Manuel Romero?Gómez, Yiguo Hu and Hong Tang

Microbiology Spectrum

Spatiotemporal observations of host-pathogen interactions in mucosa during SARS-CoV-2 infection indicate a protective role of ILC2s

作者：Wei Hu, Lu Meng, Chao Wang, Wenhan Lu, Xiaoyu Tong, Rui Lin, Tao Xu, Liang Chen, An Cui, Xiaoqing Xu, Anni Li, Jia Tang, Hongru Gao, Zhenle Pei, Ruonan Zhang, Yicong Wang, Yu Wang,Wendong Han,Ning Jiang,Chenglong Xiong,Yi Feng, Kuinyu Lee, Mingquan Chen

EMBO Reports

Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress

作者：Jing Wang, Linshan Sun, Jingjing You, Honghai Peng, Haijing Yan, Jiangong WangFengjiao Sun, Minghu Cui, Sanwang Wang, Zheng Zhang, Xueli Fan, Dunjiang Liu, Cuilan Liu, Changyun Qiu, Chao Chen, Zhicheng Xu, Jinbo Chen, Wei Li,& Bin Liu

BIOSENS BIOELECTRON

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

作者：Xiaoyan Lyu , Feiyun Cui Hang Zhou , Bo Cao , Xiaolan Zhang , Minghui Cai , Shulong Yang , Bangyong Sun , Gang Li ,

Human Brain

Study on the transparent dFC-Tesos microscopic imaging method for large tissues in human brain

作者：Di-Di Li, Xue Wang, Wen-Ying Qiu, Jing-Li Yang, Wei-Ping Wang, Xiao-Liang Wang

Nature Materials

Rapid fabrication of physically robust hydrogels

作者：Bingkun Bao, Qingmei Zeng, Kai Li, Jianfeng Wen, Yiqing Zhang, Yongjun Zheng, Renjie Zhou, Chutong Shi, Ting Chen, Chaonan Xiao, Baihang Chen, Tao Wang, Kang Yu, Yuan Sun, Qiuning Lin , Yong He, Shantung Tu & Linyong Zhu

Science China Life Science

Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

作者：Yonghui Jiang, Xueying Gao, Yue Liu, Xueqi Yan, Huangcong Shi,Rusong Zhao, Zi-Jiang Chen, Fei Gao, Han Zhao & Shigang Zhao

Cell Proliferation

Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans

作者：Meng-Chao Zhu,Wei Hu,Lei Lin Qing-Wen Yang,Lu Zhang,Jia-Lin Xu,Yi-Tong Xu, Jia-Sheng Liu,Meng-Di Zhang,Xiao-Yu Tong,Kai-Yi Zhu,Ke Feng,Yi Feng, Jian-Zhong Su,Xiu-Feng Huang,Jin Li,

The American Journal of Pathology

Defective Uterine Spiral Artery Remodeling and Placental Senescence in a Pregnant Rat Model of Polycystic Ovary Syndrome

作者：Min Hu, Yuehui Zhang, Xu Zhang, XiuYing Zhang, Xinyue Huang, Yaxing Lu, Yijia Li, Mats Br?nnstr?m,Amanda Nancy Sferruzzi-Perri, Linus . Shao, and H?kan Billigy

Cell Journal

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats after Tissue Clearing

作者：Huaixuan Sheng, , Mingru Huang, , Huizhu Li, , Luyi Sun, , Sijia Feng,, Xiner Du, Yicong Wang, Ph, Xiaoyu Tong, M.Sc., Yi Feng, M.D., Ph.D., Jun Chen, Ph.D. , Yunxia Li, M.D.

Cerebral Cortex

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats

作者：Hangqi Li, Qiuying Zhou, Yanlu Chen, Huijie Hu, Liang Gao, Toru Takahata

2022（6篇）

Cell Reports

Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection

作者：Qihang Hou, Junpeng Jia, Jian Lin, Linda Zhu, Shuang Xie, Qinghua Yu, Yuchen Li

Frontiers in Neurology

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

作者：Cheng-Feng He , Wen-Jiao Xue , Xiao-Die Xu , Jian-Tao Wang , Xin-Ru Wang ,Yi Feng, Hou-Guang Zhou and Jing-Chun Guo

Nature

Olfactory sensory experience regulates gliomagenesis via neuronal IGF1

作者：Pengxiang Chen, Wei Wang, Rui Liu, Jiahui Lyu,Lei Zhang, Baizhou Li, Biying Qiu, Anhao Tian, Wenhong Jiang, Honggang Ying, Rui Jing, Qianqian Wang, Keqing Zhu, Ruiliang Bai, Linghui Zeng, Shumin Duan& Chong Liu

Nature Communications

Carbonized paramagnetic complexes of Mn (II) as contrast agents for precise magnetic resonance imaging of sub-millimeter-sized orthotopic tumors

作者：Ruixue Qin, Shi Li, Yuwei Qiu, Yushuo Feng, Yaqing Liu, Dandan Ding, Lihua Xu, Xiaoqian Ma, Wenjing Sun& Hongmin Chen

Chinese Medicine

Three-dimensional visualization of electroacupuncture-induced activation of brown adipose tissue via sympathetic innervation in PCOS rats

作者：Hongru Gao, Xiaoyu Tong, Wei Hu, Yicong Wang, Kuinyu Lee, Xiaoqing Xu, Jiemei Shi, Zhenle Pei, Wenhan Lu, Yuning Chen, Ruonan Zhang, Zheyi Wang, Ziyu Wang, Chengzhi Han, Yu Wang and Yi Feng

Neuroscience Bulletin

Spatiotemporal Dynamics of the Molecular Expression Pattern and Intercellular Interactions in the Glial Scar Response to Spinal Cord Injury

Leilei Gong ,Yun Gu ,Xiaoxiao Han · Chengcheng Luan · Chang Liu · Xinghui Wang · Yufeng Sun · Mengru Zheng · Mengya Fang· Shuhai Yang · Lai Xu · Hualin Sun · Bin Yu · Xiaosong Gu · Songlin Zhou

2021（3篇）

Science

Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair

作者：Lingjuan He, Wenjuan Pu, Xiuxiu Liu, Zhenqian Zhang, Maoying Han, Yi Li, Xiuzhen Huang, Ximeng Han, Yan Li, Kuo Liu, Mengyang Shi, Liang Lai, Ruilin Sun, Qing-Dong Wang, Yong Ji, Jan S. Tchorz, Bin Zhou

Nanoscale

Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model

作者：Siwen Zhang, Qiyuan Chang, Pingping Li, Xiaoyu Tong, Yi Feng, Xinyao Hao, Xudong Zhang, Zhengwei Yuane and Jichun Tan

STAR Protocols

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

作者：Ruili Feng, Dongyue Wang, Yanlu Chen, Jing Lu, and Liang Gao

2020（2篇）

Cell Reports 

A Versatile Tiling Light Sheet Microscope for Cleared Tissues Imaging

作者：Yanlu Chen, Xiaoliang, Dongdong Zhang, Chunhui Wang, Ruili Feng, Xuzhao Yao Wen,1,2,12 Hao Xu, Xinyi Shirley Zhang,6 Xiao Yang, Yongyi Chen, Yi Feng, Bo Zhou, Bi-Chang Chen,Kai Lei, Shang Cai, Jie-Min Jia and Liang Gao

中國光學(xué)期刊

光片熒光顯微成像技術(shù)及應(yīng)用進(jìn)展

于湘華，劉超，柏晨，楊廷龍，彭彤，但且，閔俊偉，姚保利

C57整鼠

經(jīng)锘海組織透明化試劑盒處理，使用LS18平鋪光片顯微鏡進(jìn)行整體3D成像，可清晰觀察小鼠全身組織分布和形態(tài)，

樣本尺寸：37.3mm*77.4mm*23.1mm，

小鼠頭部細(xì)節(jié)

小鼠胸部細(xì)節(jié)

Thy1-GFP成年小鼠完整中樞神經(jīng)系統(tǒng)

采用锘海親水型透明化試劑盒制樣，分辨率：2μm×2μm×5μm

Thy1-eGFP小鼠完整顱骨腦樣本

采用锘海透明化試劑盒，使用锘海LS18平鋪光片顯微鏡進(jìn)行三維成像

神經(jīng)細(xì)節(jié)展示

 小鼠全腦血管成像                                             小鼠肺成像                           小鼠腎臟血管成像   

     乳腺腫瘤成像                       小鼠骨雙色成像                胎鼠卵巢雙色成像              小鼠睪丸血管成像

   小鼠脾臟神經(jīng)成像                   小鼠胚胎成像                     小鼠腸血管成像                   小鼠胃血管成像

更多精彩案例可在锘海視頻號觀看：